The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Transcreener ® ADP. Calcein AM is a widely used live-cell marker. Downloads. 119. 67. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. 7°C and the bottom to 36. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax 190 reader can be integrated with Molecular . (30. The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical Biology December 31, 2022 Dang-ke. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Instructions. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). SpectraMax ABS Plus SpectraTest ABS2 v1. The cuvette must be in place when you read the plate. The excitation and the emission. View online or download Molecular devices SpectraMax M5 User Manual. 0 out of 5 $ 7,699. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 0 out of 5. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Features. Auto-calibration and self-diagnostics. Typical settings for SpectraMax readers with fluorescence detection mode are shown. 0 software was used to handle data analysis and control each instrument. We would like to show you a description here but the site won’t allow us. Perkin Elmer 1420-2560 Victor X Series Plate Reader. A). Published Dec 16, 2020 | Updated Dec 16, 2020. begin reading microplates with seven mouse clicks. SpectraMax M2, M2e;. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. the reader’s stated range. . 6% for the on-board optics (monochromators). If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. , a positive control). 3. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. spr SpectraMax iD5 SpectraTest ABS2 v1. SpectraMax M2. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. 4. SpectraMax ® M2 Multi-Mode Microplate Reader. Last lab had ours for years and never had a problem with it. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. the reader’s stated range. Uses dual. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The chamber. . Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 0) OD (500 to 1100) RFU (5. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Figure 2-23: Bottle holder To install the bottle holder: 1. 313 reference gain check fail. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. This reader's dual monochromators for. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Devices’ StakMax® Microplate Stacker in a matter of minutes and . SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. SpectraMax ABS Plus SpectraTest ABS2 v1. , contact. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. • Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. S. The drawing shows a standard microplate of 15 mm height covered with a lid. 0 software was used to handle data analysis and control each instrument. Listing: #4772697. Used. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. Three data types for FP read types are also being introduced with v4. 1 SoftMax Pro v4. Fluorescent Polarization: Solid black plates, as above. Add to Cart. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. 5,112,134 5,766,875. Offer. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 3: The cuvette chamber. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Microplate Readers Biotek Synergy 2 Plate Reader Multi-Mode. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. The password is. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Microplate readers’ standard detection modes are. fluorescence). Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Published Dec 16, 2020 | Updated Dec 16, 2020. Select the lab user profile. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. In the lower half of the window, left side, there is a box labeled reader. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single 1) Select the correct plate and the wells that you want to measure. 00. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Main Menu Toggle. Equipment Details. 67. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. When a. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. . Cells in each image are identified by SoftMax Pro Software and cell-by-cell fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Molecular Devices SpectraMax M2. Warranty: 60. Description 1. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. 25 to 3. Compatible automation solutions for SpectraMax Readers. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. . plate over and over again. Temperature Control: ambient from +4 to 45C. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. Reader Documentation Version No. Figure 2. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Microplate Spectrophotometers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. as a fluorescence blank. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 00 USD. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Condition: Used - Good. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 0) OD (500 to 1100) RFU (5. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Spectramax Multi-Mode Microplate Reader Performance Specifications. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. 0) OD (500 to 1100) RFU (5. refurbished. M2. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Microplate readers’ standard detection modes. 67. Fluorescence measurements no longer require you to adjust the gain. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. g. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. 1) Select the correct plate and the wells that you want to measure. D 47 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. If you are going to read it from the bottom, you should use a clear-bottom black plate. Choose from a three- (M3), four- (M4), orThere were 12 replicate wells per dilution in both 96-well and 384- well plates. In addition, the SpectraMax iD5The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate Readers. D 47 7. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Self Service Available. 3: The cuvette chamber. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Reviews. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. Microplate Reader Shelf. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. The drawing shows a standard microplate of 15 mm height covered with a lid. td. 6 % of reading 4. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. Spectramax Multi-Mode Microplate Reader Performance Specifications. the reader’s stated range. Because. With optimized reagents, option to operate the readers. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. com 10. . The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. 316 grating motor driver faulty. ~ Bio-Tek ELx808 Microplate Reader $2,795. It allows you to explore cellular pathways and protein activation and expression in one system. Read More. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. Two important settings to obtain the best results in TRF assays. Fully tested with validations and warranty. A. Fig 2: Enzymatic response of synthetic compounds. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. In the M2, M5 and Plus. D 3 1. Plate reader set to 50 reads. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. It also. Allows you to choose your sample format depending on your unique research and throughput needs. A. The fluorometric. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. We therefore gathered salient. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. standard spectral absorbance, fluorescence, and luminescence detection. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. 2. 0077 OD 0. Standard features include a cuvette port,. Molecular Devices SpectraMax M2 Multimode Microplate Reader w/ Laptop & Software. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. 7°C and the bottom to 36. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. The plate reader optics are tunable for absorbance. M2. The plate reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. . 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. D 3 1. A . Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. BioTek Synergy 2. SpectraMax ® M2 Multi-Mode Microplate Reader. The fluorescence intensity of MitoTracker® Red CM-H2XRos was measured with a fluorescence plate reader at excitation and. Molecular Devices SpectraMax M2 Multilabel Microplate Reader. Learn how you can utilize microplate readers to drive landmark discoveries. Microplate Reader Shelf. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Fluorescence Microplate Readers 4. 2. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. 1. Driver installation. The flexibility of a high end multimode plate reader with environmental control. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Wavelength optimization. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. A solution for low volume applications. Molecular Devices SpectraMax M2 Microplate Reader. 0 out of 5 $ 16,499. 0 out of 5 $ 9,995 $ 9,495. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. In addition, the SpectraMax iD5SpectraMax Microplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Microplate Readers. 2. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Add to Cart. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. Enables accurate fluorescence data with every run. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Highly selective for dsDNA over ssDNA or RNA. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. These robust readers have been placed in labs from Antarctica to the. You should determine the expected mean OD value by reading a clean water-filled plate from each new lot. Filters: Excitation and emission slides for 4 filters each. Microplate Readers Include the M3, M4, M5, and M5. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. Read the microplate Step 1: Place the microplate in the reader. 67. VANTAstar™ Flexible Multi-mode Microplate Reader. Perform the scan and note the wavelength that gives maximum emission. Skip to main content. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 7. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. SpectraMax ® M2/M2e: Not Capable: Validated:. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). Appendix. orporation. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. SoftMax Pro. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. 1 x 38. 0 out of 5 $ 14,499 $ 13,995-13%. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. S. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. An assay plate containing a 15-point standard curve was read on the SpectraMax. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. New Life Scientific backs all orders with a 90-day warranty. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The plate is set up for reading and images are acquired according to specified parameters. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction.